Bovine Keratin- associated protein 12- 2, KRTAP12-2 ELISA KIT
Product Name:Bovine Keratin- associated protein 12- 2, KRTAP12-2 ELISA KIT
Packing:96T
Catalog No.:ELI-19293b
Gene Name:Bovine Keratin-associated protein 12-2, KRTAP12-2
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Keratin-associated protein 12-2, KRTAP12-2
Alternative Name:KRTAP12-2,Bovine Keratin-associated protein 12-2, KRTAP12-2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Keratin- associated protein 12- 2, KRTAP12-2 ELISA KIT allows for the in vitro quantitative determination of Bovine Keratin-associated protein 12-2, KRTAP12-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Keratin- associated protein 12- 2, KRTAP12-2 ELISA KIT has been pre-coated with an Bovine Keratin-associated protein 12-2, KRTAP12-2 antibody specific to Bovine Keratin-associated protein 12-2, KRTAP12-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Keratin-associated protein 12-2, KRTAP12-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Keratin-associated protein 12-2, KRTAP12-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Keratin-associated protein 12-2, KRTAP12-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!