Bovine Keratin, type I cytoskeletal 25, KRT25 ELISA KIT

Product Name:Bovine Keratin, type I cytoskeletal 25, KRT25 ELISA KIT
Packing:96T

Catalog No.:ELI-21274b

Gene Name:Bovine Keratin, type I cytoskeletal 25, KRT25

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine Keratin, type I cytoskeletal 25, KRT25

Alternative Name:KRT25,Bovine Keratin, type I cytoskeletal 25, KRT25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Keratin, type I cytoskeletal 25, KRT25 ELISA KIT allows for the in vitro quantitative determination of Bovine Keratin, type I cytoskeletal 25, KRT25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Keratin, type I cytoskeletal 25, KRT25 ELISA KIT has been pre-coated with an Bovine Keratin, type I cytoskeletal 25, KRT25 antibody specific to Bovine Keratin, type I cytoskeletal 25, KRT25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Keratin, type I cytoskeletal 25, KRT25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Keratin, type I cytoskeletal 25, KRT25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Keratin, type I cytoskeletal 25, KRT25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!