Bovine Keratin, type II cytoskeletal 75, KRT75 ELISA KIT

Product Name:Bovine Keratin, type II cytoskeletal 75, KRT75 ELISA KIT
Packing:96T

Catalog No.:ELI-08066b

Gene Name:Bovine Keratin, type II cytoskeletal 75, KRT75

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Keratin, type II cytoskeletal 75, KRT75

Alternative Name:KRT75,Bovine Keratin, type II cytoskeletal 75, KRT75
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Keratin, type II cytoskeletal 75, KRT75 ELISA KIT allows for the in vitro quantitative determination of Bovine Keratin, type II cytoskeletal 75, KRT75 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Keratin, type II cytoskeletal 75, KRT75 ELISA KIT has been pre-coated with an Bovine Keratin, type II cytoskeletal 75, KRT75 antibody specific to Bovine Keratin, type II cytoskeletal 75, KRT75 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Keratin, type II cytoskeletal 75, KRT75 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Keratin, type II cytoskeletal 75, KRT75, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Keratin, type II cytoskeletal 75, KRT75 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!