Bovine Keratocan, KERA ELISA KIT

Product Name:Bovine Keratocan, KERA ELISA KIT
Packing:96T

Catalog No.:ELI-31165b

Gene Name:Bovine Keratocan, KERA

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Bovine Keratocan, KERA

Alternative Name:KERA,Bovine Keratocan, KERA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Keratocan, KERA ELISA KIT allows for the in vitro quantitative determination of Bovine Keratocan, KERA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Keratocan, KERA ELISA KIT has been pre-coated with an Bovine Keratocan, KERA antibody specific to Bovine Keratocan, KERA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Keratocan, KERA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Keratocan, KERA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Keratocan, KERA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!