Bovine Lactadherin, MFGE8 ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Lactadherin,PAS-6/PAS-7 glycoprotein,Milk fat globule-EGF factor 8(MFG-E8),MGP57/53,MFGM,Sperm surface protein
SP47,SED1,BP47,Components 15/16,

Search name
Bovine Lactadherin ELISA KIT ,Bovine SED1 ELISA KIT ,Bovine Milk fat globule-EGF factor 8 ,Bovine MFG-E8 ELISA KIT ,Bovine
MFGM ELISA KIT ,Bovine Breast epithelial antigen BA46 ELISA KIT ,Bovine HMFG ELISA KIT ,Bovine MFGE8 ELISA KIT ,Bovine
lactadherin ELISA KIT ,Bovine BA46 ELISA KIT ,Bovine EDIL1 ELISA KIT ,Bovine HMFG ELISA KIT ,Bovine HsT19888 ELISA
KIT ,Bovine MFG-E8 ELISA KIT ,Bovine MFGM ELISA KIT ,Bovine OAcGD3S ELISA KIT ,Bovine SED1 ELISA KIT ,Bovine SPAG10
ELISA KIT ,Bovine hP47 ELISA KIT ,Bovine O-acetyl disialoganglioside synthase ELISA KIT ,Bovine breast epithelial antigen
BA46 ELISA KIT ,Bovine medin ELISA KIT ,Bovine sperm associated antigen 10 ELISA KIT ,Bovine sperm surface protein hP47
ELISA KIT ,Bovine milk fat globule-EGF factor 8 protein ELISA KIT, Bovine MGP57/53 ELISA KIT, Bovine Sperm surface protein
SP47 ELISA KIT, Bovine SED1 ELISA KIT, Bovine BP47 ELISA KIT, Bovine Components 15/16 ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Lactadherin concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactadherin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Lactadherin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Lactadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Lactadherin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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