Bovine Mitogen- activated protein kinase kinase kinase 13, MAP3K13 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MAP3K13,LZK,MEKK13; MLK; leucine zipper-bearing kinase; mixed lineage kinase; mitogen-activated protein kinase kinase kinase 13

Search name

Bovine MAP3K13 ELISA KIT ,Bovine LZK ELISA KIT ,Bovine MEKK13 ELISA KIT ,Bovine MLK ELISA KIT ,Bovine leucine zipper-bearing kinase ELISA KIT ,Bovine mixed lineage kinase ELISA KIT ,Bovine mitogen-activated protein kinase kinase kinase 13 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Mitogen- activated protein kinase kinase kinase 13, MAP3K13 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP3K13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAP3K13 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAP3K13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAP3K13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.