Bovine Matrix metalloproteinase 13, MMP13 ELISA kit

$796.00

  • Model: ELA-E0099b
  • 20 Units in Stock
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Bovine Matrix metalloproteinase 13, MMP13 ELISA kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

MMP13,CLG3; MANDP1; MMP-13; collagenase 3; matrix metallopeptidase 13; matrix metalloproteinase 13; matrix metallopeptidase 13, Matrix metalloproteinase-13, UMRCASE,

 

Search name

Bovine MMP13 ELISA KIT ,Bovine CLG3 ELISA KIT ,Bovine MANDP1 ELISA KIT ,Bovine MMP-13 ELISA KIT ,Bovine collagenase 3 ELISA KIT ,Bovine matrix metallopeptidase 13 ELISA KIT ,Bovine matrix metalloproteinase 13 ELISA KIT ,Bovine matrix metallopeptidase 13 ELISA KIT ,Bovine Matrix metalloproteinase-13 ELISA KIT ,Bovine UMRCASE ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine Collagenase 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagenase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagenase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagenase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagenase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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