Bovine Neuronal acetylcholine receptor subunit alpha- 7, CHRNA7 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CHRNA7,CHRNA7-2; NACHRA7; a7 nicotinic acetylcholine receptor; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; cholinergic receptor, nicotinic alpha 7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor protein, alpha-7 chain; cholinergic receptor nicotinic alpha 7 subunit
Search name
Bovine CHRNA7 ELISA KIT ,Bovine CHRNA7-2 ELISA KIT ,Bovine NACHRA7 ELISA KIT ,Bovine a7 nicotinic acetylcholine receptor ELISA KIT ,Bovine alpha 7 neuronal nicotinic acetylcholine receptor ELISA KIT ,Bovine alpha-7 nicotinic cholinergic receptor subunit ELISA KIT ,Bovine cholinergic receptor nicotinic alpha 7 ELISA KIT ,Bovine cholinergic receptor nicotinic alpha 7 neuronal ELISA KIT ,Bovine cholinergic receptor nicotinic alpha polypeptide 7 ELISA KIT ,Bovine neuronal acetylcholine receptor protein alpha-7 chain ELISA KIT ,Bovine cholinergic receptor nicotinic alpha 7 subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Neuronal acetylcholine receptor subunit alpha- 7, CHRNA7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bovine Neuronal acetylcholine receptor subunit alpha-7,CHRNA7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bovine Neuronal acetylcholine receptor subunit alpha-7,CHRNA7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Neuronal acetylcholine receptor subunit alpha-7,CHRNA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine Neuronal acetylcholine receptor subunit alpha-7,CHRNA7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.