Bovine NOSIP ELISA KIT

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  • Model: ELI-16703b
  • 20 Units in Stock
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Bovine NOSIP ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  NOSIP

Protein Name  E3 ubiquitin-protein ligase NOSIP

Alternative Name

Bovine NOSIP ELISA KIT , RING-type E3 ubiquitin transferase NOSIP; E3 ubiquitin-protein ligase NOSIP; CGI-25; nitric oxide synthase-interacting protein; eNOS-interacting protein; nitric oxide synthase interacting protein

 

Intended use

Bovine NOSIP ELISA KIT allows for the in vitro quantitative determination of NOSIP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Bovine NOSIP ELISA KIT has been pre-coated with an NOSIP antibody specific to NOSIP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NOSIP and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NOSIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NOSIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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