Bovine Osteocalcin/ Bone gla protein, OT/ BGP ELISA kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Osteocalcin,Bone Gla protein(BGP),Gamma-carboxyglutamic acid-containing protein,; Osteocalcin,bone gamma-carboxyglutamate (gla)
protein (osteocalcin);

Search name
Bovine Osteocalcin ELISA Kit, Bovine Bone gla protein ELISA Kit, Bovine BGP ELISA Kit, Bovine Gamma-carboxyglutamic
acid-containing protein ELISA Kit, Bovine Osteocalcin bone gamma-carboxyglutamate (gla) protein ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine osteocalcin,Osteocalcin concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Osteocalcin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Osteocalcin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteocalcin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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