Bovine Perilipin- 5, PLIN5 ELISA KIT
Product Name:Bovine Perilipin- 5, PLIN5 ELISA KIT
Packing:96T
Catalog No.:ELI-15336b
Gene Name:Bovine Perilipin-5, PLIN5
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Perilipin-5, PLIN5
Alternative Name:PLIN5,Bovine Perilipin-5, PLIN5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Perilipin- 5, PLIN5 ELISA KIT allows for the in vitro quantitative determination of Bovine Perilipin-5, PLIN5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Perilipin- 5, PLIN5 ELISA KIT has been pre-coated with an Bovine Perilipin-5, PLIN5 antibody specific to Bovine Perilipin-5, PLIN5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Perilipin-5, PLIN5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Perilipin-5, PLIN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Perilipin-5, PLIN5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!