Bovine Phosphatidylinositol- 4,5- bisphosphate 3- kinase catalyt

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  • Model: ELI-37981b
  • 20 Units in Stock
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Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform, PIK3CA ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

PIK3CA,p110,serine/threonine protein kinase PIK3CA; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; p110-alpha; PI3-kinase p110 subunit alpha; PI3K-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; phosphoinositide-3-kinase, catalytic, alpha polypeptide; ptdIns-3-kinase subunit p110-alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha

 

Search name

Bovine PIK3CA ELISA KIT ,Bovine p110 ELISA KIT ,Bovine serine/threonine protein kinase PIK3CA ELISA KIT ,Bovine CLOVE ELISA KIT ,Bovine CWS5 ELISA KIT ,Bovine MCAP ELISA KIT ,Bovine MCM ELISA KIT ,Bovine MCMTC ELISA KIT ,Bovine PI3K ELISA KIT ,Bovine p110-alpha ELISA KIT ,Bovine PI3-kinase p110 subunit alpha ELISA KIT ,Bovine PI3K-alpha ELISA KIT ,Bovine phosphatidylinositol 3-kinase catalytic110-KD alpha ELISA KIT ,Bovine phosphatidylinositol 3-kinase catalytic alpha polypeptide ELISA KIT, Bovine phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA KIT, Bovine phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA KIT, Bovine phosphoinositide-3-kinase catalytic alpha polypeptide ELISA KIT, Bovine ptdIns-3-kinase subunit p110-alpha ELISA KIT, Bovine phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ELISA KIT,

 

Intended use

Bovine PIK3CA ELISA KIT allows for the in vitro quantitative determination of Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform, PIK3CA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Bovine PIK3CA ELISA KIT has been pre-coated with an antibody specific to Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine PIK3CA ELISA KIT Range 0.15-10ng/ml

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