Bovine Phosphofructokinase ELISA Kit

Product Name:Bovine Phosphofructokinase ELISA Kit
Packing:96T

Catalog No.:ELA-E0717b

Gene Name:Bovine Phosphofructokinase

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Bovine Phosphofructokinase

Alternative Name:PFK,Bovine Phosphofructokinase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Phosphofructokinase ELISA Kit allows for the in vitro quantitative determination of Bovine Phosphofructokinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Phosphofructokinase ELISA Kit has been pre-coated with an Bovine Phosphofructokinase antibody specific to Bovine Phosphofructokinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Phosphofructokinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Phosphofructokinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Phosphofructokinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!