Bovine Phospholemman, FXYD1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

FXYD1,PLM; FXYD domain containing ion transport regulator 1

Search name

Bovine FXYD1 ELISA KIT ,Bovine PLM ELISA KIT ,Bovine FXYD domain containing ion transport regulator 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Phospholemman, FXYD1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bovine Phospholemman,FXYD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bovine Phospholemman,FXYD1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Phospholemman,FXYD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine Phospholemman,FXYD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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