Bovine POLR2F ELISA KIT

Product Name:Bovine POLR2F ELISA KIT
Packing:96T

Catalog No.:ELI-14345b

Gene Name:Bovine POLR2F

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine POLR2F

Alternative Name:POLR2F,Bovine POLR2F
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine POLR2F ELISA KIT allows for the in vitro quantitative determination of Bovine POLR2F concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine POLR2F ELISA KIT has been pre-coated with an Bovine POLR2F antibody specific to Bovine POLR2F .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine POLR2F and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine POLR2F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine POLR2F in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!