Bovine POLR3A ELISA KIT

Product Name:Bovine POLR3A ELISA KIT
Packing:96T

Catalog No.:ELI-14350b

Gene Name:Bovine POLR3A

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Bovine POLR3A

Alternative Name:POLR3A,Bovine POLR3A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine POLR3A ELISA KIT allows for the in vitro quantitative determination of Bovine POLR3A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine POLR3A ELISA KIT has been pre-coated with an Bovine POLR3A antibody specific to Bovine POLR3A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine POLR3A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine POLR3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine POLR3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!