Bovine Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HEAB,pre-mRNA cleavage complex II protein Clp1; polyribonucleotide 5-hydroxyl-kinase Clp1; polynucleotide kinase Clp1; polyadenylation factor Clp1; CLP1, cleavage and polyadenylation factor I subunit, homolog; hClp1; ATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; cleavage and polyadenylation factor I subunit 1
Search name
Bovine HEAB ELISA KIT ,Bovine pre-mRNA cleavage complex II protein Clp1 ELISA KIT ,Bovine polyribonucleotide 5-hydroxyl-kinase Clp1 ELISA KIT ,Bovine polynucleotide kinase Clp1 ELISA KIT ,Bovine polyadenylation factor Clp1 ELISA KIT ,Bovine CLP1 ELISA KIT ,Bovine cleavage and polyadenylation factor I subunit homolog ELISA KIT ,Bovine hClp1 ELISA KIT ,Bovine ATP/GTP-binding protein ELISA KIT ,Bovine homolog of yeast CFIA subunit Clp1p ELISA KIT ,Bovine cleavage and polyadenylation factor I subunit 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Polyribonucleotide 5’- hydroxyl- kinase Clp1, CLP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CLP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CLP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.