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  • Model: ELI-51733b
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Bovine 2- oxo- 4- hydroxy- 4- carboxy- 5- ureidoimidazoline decarboxylase, PRHOXNB ELISA KIT

96 Tests

Operating instruction



Alternative name

Bovine PRHOXNB ELISA KIT ,Bovine URAD ELISA KIT ,Bovine OHCU decarboxylase ELISA KIT ,Bovine parahox cluster neighbor ELISA KIT ,Bovine parahox neighbor ELISA KIT ,Bovine ureidoimidazoline ELISA KIT ,Bovine 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase ELISA KIT ,Bovine Putative 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase ELISA KIT


Intended use

Bovine PRHOXNB kit allows for the in vitro quantitative determination of Bovine 2-oxo-4-hydroxy-4-carboxy- 5-ureidoimidazoline decarboxylase, PRHOXNB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRHOXNB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PRHOXNB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PRHOXNB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PRHOXNB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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