Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 ELISA KIT

Product Name:Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 ELISA KIT
Packing:96T

Catalog No.:ELI-37730b

Gene Name:Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3

Detect Range:3.125-200ng/ml

Sensitivity:1.875ng/ml

Target Protein Name:Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3

Alternative Name:PTGER3,Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 ELISA KIT allows for the in vitro quantitative determination of Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 ELISA KIT has been pre-coated with an Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 antibody specific to Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Prostaglandin E2 receptor EP3 subtype, PTGER3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!