Bovine Protein disulfide- isomerase A3, PDIA3 ELISA KIT

Product Name:Bovine Protein disulfide- isomerase A3, PDIA3 ELISA KIT
Packing:96T

Catalog No.:ELI-04941b

Gene Name:Bovine Protein disulfide-isomerase A3, PDIA3

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Bovine Protein disulfide-isomerase A3, PDIA3

Alternative Name:PDIA3,Bovine Protein disulfide-isomerase A3, PDIA3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Protein disulfide- isomerase A3, PDIA3 ELISA KIT allows for the in vitro quantitative determination of Bovine Protein disulfide-isomerase A3, PDIA3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Protein disulfide- isomerase A3, PDIA3 ELISA KIT has been pre-coated with an Bovine Protein disulfide-isomerase A3, PDIA3 antibody specific to Bovine Protein disulfide-isomerase A3, PDIA3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Protein disulfide-isomerase A3, PDIA3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Protein disulfide-isomerase A3, PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Protein disulfide-isomerase A3, PDIA3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!