Bovine Protein disulfide- isomerase A5, PDIA5 ELISA KIT
Product Name:Bovine Protein disulfide- isomerase A5, PDIA5 ELISA KIT
Packing:96T
Catalog No.:ELI-37722b
Gene Name:Bovine Protein disulfide-isomerase A5, PDIA5
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Protein disulfide-isomerase A5, PDIA5
Alternative Name:PDIA5,Bovine Protein disulfide-isomerase A5, PDIA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Protein disulfide- isomerase A5, PDIA5 ELISA KIT allows for the in vitro quantitative determination of Bovine Protein disulfide-isomerase A5, PDIA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Protein disulfide- isomerase A5, PDIA5 ELISA KIT has been pre-coated with an Bovine Protein disulfide-isomerase A5, PDIA5 antibody specific to Bovine Protein disulfide-isomerase A5, PDIA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Protein disulfide-isomerase A5, PDIA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Protein disulfide-isomerase A5, PDIA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Protein disulfide-isomerase A5, PDIA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!