Bovine Putative phospholipase B-like 2, Plbd2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Bovine PLBD2 ELISA KIT ,Bovine P76 ELISA KIT ,Bovine 76 kDa protein ELISA KIT ,Bovine LAMA-like protein 2 ELISA KIT ,Bovine PLB homolog 2 ELISA KIT ,Bovine lamina ancestor homolog 2 ELISA KIT ,Bovine mannose-6-phosphate protein associated protein p76 ELISA KIT ,Bovine phospholipase B domain-containing protein 2 ELISA KIT ,Bovine phospholipase B-like 2 32 kDa form ELISA KIT ,Bovine phospholipase B-like 2 45 kDa form ELISA KIT ,Bovine phospholipase B domain containing 2 ELISA KIT ,Bovine Putative phospholipase B-like 2 ELISA KIT ,Bovine 66.3 kDa protein ELISA KIT

Intended use

Bovine Plbd2 ELISA kit allows for the in vitro quantitative determination of Bovine Putative phospholipase B-like 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Bovine Plbd2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plbd2, During the reaction, Plbd2 in the sample or standard competes with a fixed amount of biotin-labeled Plbd2 for sites on a pre-coated Monoclonal antibody specific to Plbd2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Plbd2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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