Bovine Recoverin, RCVRN ELISA KIT
Product Name:Bovine Recoverin, RCVRN ELISA KIT
Packing:96T
Catalog No.:ELI-18222b
Gene Name:Bovine Recoverin, RCVRN
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Recoverin, RCVRN
Alternative Name:RCVRN,Bovine Recoverin, RCVRN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Recoverin, RCVRN ELISA KIT allows for the in vitro quantitative determination of Bovine Recoverin, RCVRN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Recoverin, RCVRN ELISA KIT has been pre-coated with an Bovine Recoverin, RCVRN antibody specific to Bovine Recoverin, RCVRN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Recoverin, RCVRN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Recoverin, RCVRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Recoverin, RCVRN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!