Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT

Product Name:Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT
Packing:96T

Catalog No.:ELI-20292b

Gene Name:Bovine Ribonuclease H2 subunit B, RNASEH2B

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Ribonuclease H2 subunit B, RNASEH2B

Alternative Name:RNASEH2B,Bovine Ribonuclease H2 subunit B, RNASEH2B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT allows for the in vitro quantitative determination of Bovine Ribonuclease H2 subunit B, RNASEH2B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Ribonuclease H2 subunit B, RNASEH2B ELISA KIT has been pre-coated with an Bovine Ribonuclease H2 subunit B, RNASEH2B antibody specific to Bovine Ribonuclease H2 subunit B, RNASEH2B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ribonuclease H2 subunit B, RNASEH2B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ribonuclease H2 subunit B, RNASEH2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ribonuclease H2 subunit B, RNASEH2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!