Bovine RING finger protein 114, RNF114 ELISA KIT
Product Name:Bovine RING finger protein 114, RNF114 ELISA KIT
Packing:96T
Catalog No.:ELI-42642b
Gene Name:Bovine RING finger protein 114, RNF114
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine RING finger protein 114, RNF114
Alternative Name:RNF114,Bovine RING finger protein 114, RNF114
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine RING finger protein 114, RNF114 ELISA KIT allows for the in vitro quantitative determination of Bovine RING finger protein 114, RNF114 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine RING finger protein 114, RNF114 ELISA KIT has been pre-coated with an Bovine RING finger protein 114, RNF114 antibody specific to Bovine RING finger protein 114, RNF114 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine RING finger protein 114, RNF114 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine RING finger protein 114, RNF114, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine RING finger protein 114, RNF114 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!