Bovine Thiamine- triphosphatase, THTPA ELISA KIT

Product Name:Bovine Thiamine- triphosphatase, THTPA ELISA KIT
Packing:96T

Catalog No.:ELI-40005b

Gene Name:Bovine Thiamine-triphosphatase, THTPA

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Bovine Thiamine-triphosphatase, THTPA

Alternative Name:THTPA,Bovine Thiamine-triphosphatase, THTPA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Thiamine- triphosphatase, THTPA ELISA KIT allows for the in vitro quantitative determination of Bovine Thiamine-triphosphatase, THTPA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Thiamine- triphosphatase, THTPA ELISA KIT has been pre-coated with an Bovine Thiamine-triphosphatase, THTPA antibody specific to Bovine Thiamine-triphosphatase, THTPA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Thiamine-triphosphatase, THTPA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Thiamine-triphosphatase, THTPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Thiamine-triphosphatase, THTPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!