Bovine Transcription factor IIIB 50 kDa subunit, BRF2 ELISA KIT

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  • Model: ELI-25351b
  • 20 Units in Stock
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Bovine Transcription factor IIIB 50 kDa subunit, BRF2 ELISA KIT

Product Name:Bovine Transcription factor IIIB 50 kDa subunit, BRF2 ELISA KIT
Packing:96T

Catalog No.:ELI-25351b

Gene Name:Bovine Transcription factor IIIB 50 kDa subunit, BRF2

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Bovine Transcription factor IIIB 50 kDa subunit, BRF2

Alternative Name:BRF2,Bovine Transcription factor IIIB 50 kDa subunit, BRF2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Transcription factor IIIB 50 kDa subunit, BRF2 ELISA KIT allows for the in vitro quantitative determination of Bovine Transcription factor IIIB 50 kDa subunit, BRF2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Transcription factor IIIB 50 kDa subunit, BRF2 ELISA KIT has been pre-coated with an Bovine Transcription factor IIIB 50 kDa subunit, BRF2 antibody specific to Bovine Transcription factor IIIB 50 kDa subunit, BRF2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Transcription factor IIIB 50 kDa subunit, BRF2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Transcription factor IIIB 50 kDa subunit, BRF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Transcription factor IIIB 50 kDa subunit, BRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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