Bovine Transketolase, TKT ELISA Kit


  • Model: ELA-E0113b
  • 20 Units in Stock
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Bovine Transketolase, TKT ELISA Kit


96 Tests


Alternative name

Bovine TKT ELISA KIT ,Bovine TK ELISA KIT ,Bovine Transketolase ELISA KIT ,Bovine HEL-S-48 ELISA KIT ,Bovine HEL107 ELISA KIT ,Bovine TKT1 ELISA KIT ,Bovine epididymis luminal protein 107 ELISA KIT ,Bovine epididymis secretory protein Li 48 ELISA KIT


Intended use

Bovine TKT ELISA kit allows for the in vitro quantitative determination of Bovine TKT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in Bovine TKT ELISA kit has been pre-coated with an antibody specific to TKT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TKT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TKT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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