Bovine UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L1, UCHL1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  UCHL1

Protein Name  UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1

Alternative Name

Bovine UCHL1 ELISA KIT ,Bovine HEL-117 ELISA KIT ,Bovine HEL-S-53 ELISA KIT ,Bovine NDGOA ELISA KIT ,Bovine PARK5 ELISA KIT ,Bovine PGP 9.5 ELISA KIT ,Bovine PGP9.5 ELISA KIT ,Bovine PGP95 ELISA KIT ,Bovine SPG79 ELISA KIT ,Bovine Uch-L1 ELISA KIT ,Bovine ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA KIT ,Bovine epididymis luminal protein 117 ELISA KIT ,Bovine epididymis secretory protein Li 53 ELISA KIT ,Bovine neuron cytoplasmic protein 9.5 ELISA KIT ,Bovine ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA KIT ,Bovine ubiquitin thioesterase L1 ELISA KIT ,Bovine ubiquitin thiolesterase ELISA KIT ,Bovine ubiquitin C-terminal hydrolase L1 ELISA KIT

Intended use

Bovine UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 ELISA KIT allows for the in vitro quantitative determination of UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Bovine UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 ELISA KIT has been pre-coated with an UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 antibody specific to UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBIQUITIN CARBOXYL- TERMINAL HYDROLASE ISOZYME L1, UCHL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20