Bovine Ubiquitin- like protein ATG12, ATG12 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Ubiquitin-like protein ATG12,Autophagy-related protein 12,APG12-like, ATG12,ubiquitin-like protein ATG12; ATG12 autophagy related 12 homolog; APG12; APG12L; FBR93; HAPG12; Apg12 (autophagy, yeast) homolog; autophagy related 12
Search name’
Bovine Ubiquitin-like protein ATG12 ELISA KIT ,Bovine Autophagy-related protein 12 ELISA KIT ,Bovine APG12-like ELISA KIT ,Bovine ATG12 ELISA KIT ,Bovine ubiquitin-like protein ATG12 ELISA KIT ,Bovine ATG12 autophagy related 12 homolog ELISA KIT ,Bovine APG12 ELISA KIT ,Bovine APG12L ELISA KIT ,Bovine FBR93 ELISA KIT ,Bovine HAPG12 ELISA KIT ,Bovine Apg12 (autophagy ELISA KIT ,Bovine yeast) homolog ELISA KIT ,Bovine autophagy related 12 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Ubiquitin-like protein ATG12 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ATG12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ATG12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ATG12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin-like protein ATG12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.