Bovine Ubiquitin- like protein ISG15, ISG15 ELISA KIT

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  • Model: ELI-28057b
  • 20 Units in Stock
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Bovine Ubiquitin- like protein ISG15, ISG15 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Ubiquitin-like protein ISG15,; Ubiquitin cross-reactive protein,Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein, ISG15,ISG15 ubiquitin-like modifier; G1P2; IFI15; IP17; UCRP; hUCRP; interferon alpha-inducible protein clone IFI-15K;

interferon-stimulated protein 15 kDa;

 

Search name

Bovine Ubiquitin-like protein ISG15 ELISA KIT , Bovine Ubiquitin cross-reactive protein ELISA KIT ,Bovine Interferon-induced 15 kDa protein ELISA KIT ,Bovine Interferon-induced 17 kDa protein ELISA KIT ,Bovine  ISG15 ELISA KIT ,Bovine ISG15 ubiquitin-like modifier ELISA KIT ,Bovine G1P2 ELISA KIT ,Bovine IFI15 ELISA KIT ,Bovine IP17 ELISA KIT ,Bovine UCRP ELISA KIT ,Bovine hUCRP ELISA KIT ,Bovine interferon alpha-inducible protein clone IFI-15K ELISA KIT ,Bovine interferon-stimulated protein 15 kDa ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Ubiquitin-like protein ISG15 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ISG15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ISG15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin-like protein ISG15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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