Bovine Uracil phosphoribosyltransferase homolog, UPRT ELISA KIT
Product Name:Bovine Uracil phosphoribosyltransferase homolog, UPRT ELISA KIT
Packing:96T
Catalog No.:ELI-22246b
Gene Name:Bovine Uracil phosphoribosyltransferase homolog, UPRT
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Uracil phosphoribosyltransferase homolog, UPRT
Alternative Name:UPRT,Bovine Uracil phosphoribosyltransferase homolog, UPRT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Uracil phosphoribosyltransferase homolog, UPRT ELISA KIT allows for the in vitro quantitative determination of Bovine Uracil phosphoribosyltransferase homolog, UPRT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Uracil phosphoribosyltransferase homolog, UPRT ELISA KIT has been pre-coated with an Bovine Uracil phosphoribosyltransferase homolog, UPRT antibody specific to Bovine Uracil phosphoribosyltransferase homolog, UPRT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Uracil phosphoribosyltransferase homolog, UPRT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Uracil phosphoribosyltransferase homolog, UPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Uracil phosphoribosyltransferase homolog, UPRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!