Canine 17- Hydroxyprogesterone, 17- OHP ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
17-Hydroxyprogesterone17-OH progesterone,17-OHP

Search name
Canine 17-Hydroxyprogesterone ELISA Kit, Canine 17-OHP ELISA Kit,17-Hydroxyprogesterone ELISA Kit,17-OHP ELISA Kit,
ELISA Kit, 17-Hydroxyprogesterone17-OH progesterone,17-OHP

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general 17-hydroxyprogesterone,17-Hydroxyprogesterone
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 17-Hydroxyprogesterone, During the reaction, 17-Hydroxyprogesterone in the sample or standard
competes with a fixed amount of biotin-labeled 17-Hydroxyprogesterone for sites on a pre-coated Monoclonal antibody specific to
17-Hydroxyprogesterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 17-Hydroxyprogesterone in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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