Canine Beta lactoglobulin, Beta-LG ELISA Kit

Product Name:Canine Beta lactoglobulin, Beta-LG ELISA Kit
Packing:96T

Catalog No.:ELA-E1023d

Gene Name:Canine Beta lactoglobulin, Beta-LG

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Beta lactoglobulin, Beta-LG

Alternative Name:Beta lactoglobulin,Beta lactoglobulin, Beta-LG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Beta lactoglobulin, Beta-LG ELISA Kit allows for the in vitro quantitative determination of Canine Beta lactoglobulin, Beta-LG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Beta lactoglobulin, Beta-LG ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Beta-lactoglobulin,Beta-LG,LGB,Beta lactoglobulin,Beta LG

 

Search name

Canine Beta-lactoglobulin ELISA KIT ,Canine Beta-LG ELISA KIT ,Canine LGB ELISA KIT ,Canine Beta lactoglobulin ELISA KIT ,Canine Beta LG ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine beta-lactoglobulin,Beta-LG concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-LG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-LG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-LG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Beta-LG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Beta lactoglobulin, Beta-LG antibody specific to Canine Beta lactoglobulin, Beta-LG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Beta lactoglobulin, Beta-LG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Beta lactoglobulin, Beta-LG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Beta lactoglobulin, Beta-LG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!