Canine Chymase, CMA1 ELISA KIT
Product Name:Canine Chymase, CMA1 ELISA KIT
Packing:96T
Catalog No.:ELI-06605d
Gene Name:Canine Chymase, CMA1
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine Chymase, CMA1
Alternative Name:Chymase,Chymase, CMA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Chymase, CMA1 ELISA KIT allows for the in vitro quantitative determination of Canine Chymase, CMA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Chymase, CMA1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Chymase,Mast cell protease I,Mast cell protease 5(mMCP-5),Alpha-chymase,Mast cell chymase 1, CMA1,Chymase,chymase 1, mast cell; chymase, mast cell; chymase, heart; chymase 1 preproprotein transcript I; chymase 1 preproprotein transcript E; alpha-chymase; chymase; CYH; MCT1; mast cell protease I
Search name
Canine CMA1 ELISA KIT ,Canine Chymase ELISA KIT ,Canine chymase 1 ELISA KIT ,Canine mast cell ELISA KIT ,Canine chymase mast cell ELISA KIT ,Canine chymase heart ELISA KIT ,Canine chymase 1 preproprotein transcript I ELISA KIT ,Canine chymase 1 preproprotein transcript E ELISA KIT ,Canine alpha-chymase ELISA KIT ,Canine chymase ELISA KIT ,Canine CYH ELISA KIT ,Canine MCT1 ELISA KIT ,Canine mast cell protease I ELISA KIT, Canine CYH ELISA KIT, Canine CYM ELISA KIT, Canine Alpha-chymase ELISA KIT, Canine MCPT5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of canine Mast cell protease I concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mast cell protease I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mast cell protease I and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mast cell protease I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mast cell protease I in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine Chymase, CMA1 antibody specific to Canine Chymase, CMA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Chymase, CMA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Chymase, CMA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Chymase, CMA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!