Canine Collagen alpha-1(I) chain, COL1A1 ELISA KIT

Product Name:Canine Collagen alpha-1(I) chain, COL1A1 ELISA KIT
Packing:96T

Catalog No.:ELI-02034d

Gene Name:Canine Collagen alpha-1(I) chain, COL1A1

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Collagen alpha-1(I) chain, COL1A1

Alternative Name:Collagen alpha-1(I) chain,Collagen alpha-1(I) chain, COL1A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Collagen alpha-1(I) chain, COL1A1 ELISA KIT allows for the in vitro quantitative determination of Canine Collagen alpha-1(I) chain, COL1A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Collagen alpha-1(I) chain, COL1A1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

COL1A1,Collagen alpha-1(I) chain,Alpha-1 type I collagen, type I collagen,EDSC; OI1; OI2; OI3; OI4; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone alpha-1 chain; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain; collagen type I alpha 1

 

Search name

Canine COL1A1 ELISA KIT ,Canine Collagen alpha-1(I) chain ELISA KIT ,Canine Alpha-1 type I collagen ELISA KIT ,Canine  type I collagen ELISA KIT ,Canine EDSC ELISA KIT ,Canine OI1 ELISA KIT ,Canine OI2 ELISA KIT ,Canine OI3 ELISA KIT ,Canine OI4 ELISA KIT ,Canine alpha-1 type I collagen ELISA KIT ,Canine alpha1(I) procollagen ELISA KIT ,Canine collagen alpha 1 chain type I ELISA KIT ,Canine collagen alpha-1(I) chain preproprotein ELISA KIT ,Canine collagen of skin ELISA KIT ,Canine  tendon and bone alpha-1 chain ELISA KIT ,Canine pro-alpha-1 collagen type 1 ELISA KIT ,Canine type I proalpha 1 ELISA KIT ,Canine type I procollagen alpha 1 chain ELISA KIT ,Canine collagen type I alpha 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of canine Collagen alpha-1(I) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen alpha-1(I) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagen alpha-1(I) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagen alpha-1(I) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagen alpha-1(I) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Collagen alpha-1(I) chain, COL1A1 antibody specific to Canine Collagen alpha-1(I) chain, COL1A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Collagen alpha-1(I) chain, COL1A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Collagen alpha-1(I) chain, COL1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Collagen alpha-1(I) chain, COL1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!