Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD ELISA KIT

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  • Model: ELI-48818d
  • 20 Units in Stock
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Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD ELISA KIT

Product Name:Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD ELISA KIT
Packing:96T

Catalog No.:ELI-48818d

Gene Name:Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD

Alternative Name:Dihydrolipoyl dehydrogenase mitochondrial,Dihydrolipoyl dehydrogenase mitochondrial, DLD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD ELISA KIT allows for the in vitro quantitative determination of Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Dihydrolipoyl dehydrogenase mitochondrial, Dld ELISA KIT

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Dihydrolipoyl dehydrogenase mitochondrial,Dihydrolipoamide dehydrogenase, Dld,GCSL,LAD,PHE3,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLDD,DLDH,GCSL,LAD,PHE3,E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex,diaphorase,dihydrolipoyl dehydrogenase, mitochondrial,glycine cleavage system L protein,glycine cleavage system protein L,lipoamide dehydrogenase,lipoamide reductase,lipoyl dehydrogenase

 

Search name

Canine Dihydrolipoyl dehydrogenase mitochondrial ELISA KIT ,Canine Dihydrolipoamide dehydrogenase ELISA KIT ,Canine Dld ELISA KIT ,Canine GCSL ELISA KIT ,Canine LAD ELISA KIT ,Canine PHE3 ELISA KIT ,Canine Dihydrolipoamide dehydrogenase ELISA KIT ,Canine Glycine cleavage system L protein ELISA KIT ,Canine DLDD ELISA KIT ,Canine DLDH ELISA KIT ,Canine GCSL ELISA KIT ,Canine LAD ELISA KIT ,Canine PHE3 ELISA KIT ,Canine E3 component of pyruvate dehydrogenase complex ELISA KIT ,Canine 2-oxo-glutarate complex ELISA KIT ,Canine branched chain keto acid dehydrogenase complex ELISA KIT ,Canine diaphorase ELISA KIT ,Canine dihydrolipoyl dehydrogenase ELISA KIT ,Canine mitochondrial ELISA KIT ,Canine glycine cleavage system L protein ELISA KIT ,Canine glycine cleavage system protein L ELISA KIT ,Canine lipoamide dehydrogenase ELISA KIT ,Canine lipoamide reductase ELISA KIT ,Canine lipoyl dehydrogenase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine dihydrolipoyl dehydrogenase, mitochondrial,DLDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLDH, During the reaction, DLDH in the sample or standard competes with a fixed amount of biotin-labeled DLDH for sites on a pre-coated Monoclonal antibody specific to DLDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DLDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD antibody specific to Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Dihydrolipoyl dehydrogenase mitochondrial, DLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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