Canine Ferritin heavy chain, FTH1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ferritin heavy chain,Ferritin H subunit,Cell proliferation-inducing gene 15 protein, FTH1,FHC; FTH; FTHL6; PIG15; PLIF; apoferritin; cell proliferation-inducing gene 15 protein; ferritin H subunit; ferritin heavy chain; placenta immunoregulatory factor; proliferation-inducing protein 15; ferritin, heavy polypeptide 1

Search name

Canine Ferritin heavy chain ELISA KIT ,Canine Ferritin H subunit ELISA KIT ,Canine Cell proliferation-inducing gene 15 protein ELISA KIT ,Canine  FTH1 ELISA KIT ,Canine FHC ELISA KIT ,Canine FTH ELISA KIT ,Canine FTHL6 ELISA KIT ,Canine PIG15 ELISA KIT ,Canine PLIF ELISA KIT ,Canine apoferritin ELISA KIT ,Canine cell proliferation-inducing gene 15 protein ELISA KIT ,Canine ferritin H subunit ELISA KIT ,Canine ferritin heavy chain ELISA KIT ,Canine placenta immunoregulatory factor ELISA KIT ,Canine proliferation-inducing protein 15 ELISA KIT ,Canine ferritin heavy polypeptide 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine ferritin heavy chain,Ferritin H subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin H subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ferritin H subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ferritin H subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ferritin H subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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