Canine GAPDH ELISA KIT
Product Name:Canine GAPDH ELISA KIT
Packing:96T
Catalog No.:ELI-06211d
Gene Name:Canine GAPDH
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine GAPDH
Alternative Name:GAPDH,GAPDH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine GAPDH ELISA KIT allows for the in vitro quantitative determination of Canine GAPDH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine GAPDH ELISA KIT has been pre-coated with an Canine GAPDH antibody specific to Canine GAPDH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine GAPDH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine GAPDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine GAPDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!