Canine GJA5 ELISA KIT

Product Name:Canine GJA5 ELISA KIT
Packing:96T

Catalog No.:ELI-09906d

Gene Name:Canine GJA5

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Canine GJA5

Alternative Name:Gap junction alpha-5 protein,GJA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine GJA5 ELISA KIT allows for the in vitro quantitative determination of Canine GJA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine GJA5 ELISA KIT has been pre-coated with an Canine GJA5 antibody specific to Canine GJA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine GJA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine GJA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine GJA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!