Canine Glutamate decarboxylase 1, GAD1 ELISA KIT
Product Name:Canine Glutamate decarboxylase 1, GAD1 ELISA KIT
Packing:96T
Catalog No.:ELI-04162d
Gene Name:Canine Glutamate decarboxylase 1, GAD1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine Glutamate decarboxylase 1, GAD1
Alternative Name:Glutamate decarboxylase 1,Glutamate decarboxylase 1, GAD1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Glutamate decarboxylase 1, GAD1 ELISA KIT allows for the in vitro quantitative determination of Canine Glutamate decarboxylase 1, GAD1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Glutamate decarboxylase 1, GAD1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamate decarboxylase 1,67 kDa glutamic acid decarboxylase,GAD-67,Glutamate decarboxylase 67 kDa isoform, GAD1,glutamate decarboxylase 1 brain 67kDa; glutamate decarboxylase 1; CPSQ1; GAD; SCP; 67 kDa glutamic acid decarboxylase; GAD-67; glutamate decarboxylase 67 kDa isoform
Search name
Canine Glutamate decarboxylase 1 ELISA KIT ,Canine 67 kDa glutamic acid decarboxylase ELISA KIT ,Canine GAD-67 ELISA KIT ,Canine Glutamate decarboxylase 67 kDa isoform ELISA KIT ,Canine GAD1 ELISA KIT ,Canine glutamate decarboxylase 1 brain 67kDa ELISA KIT ,Canine glutamate decarboxylase 1 ELISA KIT ,Canine CPSQ1 ELISA KIT ,Canine GAD ELISA KIT ,Canine SCP ELISA KIT ,Canine 67 kDa glutamic acid decarboxylase ELISA KIT ,Canine GAD-67 ELISA KIT ,Canine glutamate decarboxylase 67 kDa isoform ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Glutamate decarboxylase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate decarboxylase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glutamate decarboxylase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate decarboxylase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutamate decarboxylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine Glutamate decarboxylase 1, GAD1 antibody specific to Canine Glutamate decarboxylase 1, GAD1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Glutamate decarboxylase 1, GAD1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Glutamate decarboxylase 1, GAD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Glutamate decarboxylase 1, GAD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!