Canine Glycogen debranching enzyme, GDE ELISA Kit

Product Name:Canine Glycogen debranching enzyme, GDE ELISA Kit
Packing:96T

Catalog No.:ELI-30967d

Gene Name:Canine Glycogen debranching enzyme, GDE

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Glycogen debranching enzyme, GDE

Alternative Name:Glycogen debranching enzyme,Glycogen debranching enzyme, GDE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Glycogen debranching enzyme, GDE ELISA Kit allows for the in vitro quantitative determination of Canine Glycogen debranching enzyme, GDE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Glycogen debranching enzyme, GDE ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

GDE,AGL,glycogen debranching enzyme,amylo-1, 6-glucosidase, 4-alpha-glucanotransferase; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided; glycogen debrancher; glycogen debranching protein; amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

 

Search name

Canine GDE ELISA KIT,Canine AGL ELISA KIT,Canine glycogen debranching enzyme ELISA KIT,Canine amylo-1 ELISA KIT,Canine 6-glucosidase 4-alpha-glucanotransferase ELISA KIT,Canine glycogen debrancher ELISA KIT,Canine glycogen debranching protein ELISA KIT,Canine amylo-alpha-1, 6-glucosidase 4-alpha-glucanotransferase ELISA KIT, Canine amylo-alpha-1, 6-glucosidase 4-alpha-glucanotransferaseprovided ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofCanine Glycogen debranching enzymeconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen debranching enzyme. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGlycogen debranching enzyme and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen debranching enzyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycogen debranching enzyme in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Glycogen debranching enzyme, GDE antibody specific to Canine Glycogen debranching enzyme, GDE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Glycogen debranching enzyme, GDE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Glycogen debranching enzyme, GDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Glycogen debranching enzyme, GDE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!