Canine Growth/differentiation factor 8, GDF8 ELISA Kit
Product Name:Canine Growth/differentiation factor 8, GDF8 ELISA Kit
Packing:96T
Catalog No.:ELI-05410d
Gene Name:Canine Growth(differentiation factor 8, GDF8
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine Growth(differentiation factor 8, GDF8
Alternative Name:Growth/ differentiation factor 8,Growth(differentiation factor 8, GDF8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Growth/differentiation factor 8, GDF8 ELISA Kit allows for the in vitro quantitative determination of Canine Growth(differentiation factor 8, GDF8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Growth/differentiation factor 8,GDF8 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MSTN,myostatin; GDF8; MSLHP,Growth/differentiation factor 8, GDF-8
Search name
Canine MSTN ELISA KIT,Canine myostatin ELISA KIT,Canine GDF8 ELISA KIT,Canine MSLHP ELISA KIT, Canine Growth/differentiation factor 8 ELISA KIT,Canine GDF-8 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of canine growth/differentiation factor 8,GDF-8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GDF-8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GDF-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GDF-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine Growth(differentiation factor 8, GDF8 antibody specific to Canine Growth(differentiation factor 8, GDF8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Growth(differentiation factor 8, GDF8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Growth(differentiation factor 8, GDF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Growth(differentiation factor 8, GDF8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!