Canine GUCY1A3 ELISA KIT
Product Name:Canine GUCY1A3 ELISA KIT
Packing:96T
Catalog No.:ELI-47193d
Gene Name:Canine GUCY1A3
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine GUCY1A3
Alternative Name:GUCY1A3,GUCY1A3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine GUCY1A3 ELISA KIT allows for the in vitro quantitative determination of Canine GUCY1A3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine GUCY1A3 ELISA KIT has been pre-coated with an Canine GUCY1A3 antibody specific to Canine GUCY1A3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine GUCY1A3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine GUCY1A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine GUCY1A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!