Canine HC II ELISA Kit

Product Name:Canine HC II ELISA Kit
Packing:96T

Catalog No.:ELA-E0284d

Gene Name:Canine HC II

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine HC II

Alternative Name:heparin cofactor II,HC II
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine HC II ELISA Kit allows for the in vitro quantitative determination of Canine HC II concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine HC II ELISA Kit has been pre-coated with an Canine HC II antibody specific to Canine HC II .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine HC II and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine HC II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine HC II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!