Canine HPRT1 ELISA Kit
Product Name:Canine HPRT1 ELISA Kit
Packing:96T
Catalog No.:ELI-20315d
Gene Name:Canine HPRT1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine HPRT1
Alternative Name:HPRT1,HPRT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine HPRT1 ELISA Kit allows for the in vitro quantitative determination of Canine HPRT1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Hypoxanthine- guanine phosphoribosyltransferase, HPRT1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HPRT1,HGPRT; HPRT; HGPRTase; hypoxanthine-guanine phosphoribosyltransferase 1; hypoxanthine phosphoribosyltransferase 1, Hypoxanthine-guanine phosphoribosyltransferase
Search name
Canine HPRT1 ELISA KIT ,Canine HGPRT ELISA KIT ,Canine HPRT ELISA KIT ,Canine HGPRTase ELISA KIT ,Canine hypoxanthine-guanine phosphoribosyltransferase 1 ELISA KIT ,Canine hypoxanthine phosphoribosyltransferase 1 ELISA KIT ,Canine Hypoxanthine-guanine phosphoribosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of canine hypoxanthine-guanine phosphoribosyltransferase,HGPRT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGPRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HGPRT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HGPRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HGPRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine HPRT1 antibody specific to Canine HPRT1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine HPRT1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine HPRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine HPRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!