Canine HSD3B ELISA KIT

Product Name:Canine HSD3B ELISA KIT
Packing:96T

Catalog No.:ELI-35044d

Gene Name:Canine HSD3B

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine HSD3B

Alternative Name:HSD3B,HSD3B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine HSD3B ELISA KIT allows for the in vitro quantitative determination of Canine HSD3B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine 3 beta- hydroxysteroid dehydrogenase/Delta 5- - >4- isomerase, HSD3B ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

HSD3B,HSD3B1,3BETAHSD; HSDB3; HSDB3A; SDR11E1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; delta-5-3-ketosteroid isomerase; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; progesterone reductase; short chain dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; trophoblast antigen FDO161G; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

 

Search name

Canine HSD3B ELISA KIT ,Canine HSD3B1 ELISA KIT ,Canine 3BETAHSD ELISA KIT ,Canine HSDB3 ELISA KIT ,Canine HSDB3A ELISA KIT ,Canine SDR11E1 ELISA KIT ,Canine 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA KIT ,Canine 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I ELISA KIT ,Canine 3-beta-HSD I ELISA KIT ,Canine 3-beta-hydroxy-5-ene steroid dehydrogenase ELISA KIT ,Canine 3-beta-hydroxy-Delta(5)-steroid dehydrogenase ELISA KIT ,Canine delta-5-3-ketosteroid isomerase ELISA KIT ,Canine hydroxy-delta-5-steroid dehydrogenase ELISA KIT ,Canine  3 beta-and steroid delta-isomerase 1 ELISA KIT ,Canine progesterone reductase ELISA KIT ,Canine short chain dehydrogenase/reductase family 11E ELISA KIT ,Canine  member 1 ELISA KIT ,Canine steroid Delta-isomerase ELISA KIT ,Canine trophoblast antigen FDO161G ELISA KIT ,Canine hydroxy-delta-5-steroid dehydrogenase ELISA KIT ,Canine 3 beta- and steroid delta-isomerase 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine 3 beta- hydroxysteroid dehydrogenase/Delta 5- - >4- isomerase, HSD3B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSD3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSD3B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HSD3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HSD3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Customized Product

has been pre-coated with an Canine HSD3B antibody specific to Canine HSD3B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine HSD3B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine HSD3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine HSD3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!