Canine inositol 1,4,5,-trisphosphate, IP3 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Inositol trisphosphate,Inositol 1,4,5-trisphosphate,IP3

Search name
Canine inositol 1,4,5,-trisphosphate ELISA Kit, Canine Inositol trisphosphate ELISA Kit, Canine IP3 ELISA Kit,inositol
1,4,5,-trisphosphate ELISA Kit,Inositol trisphosphate ELISA Kit,IP3 ELISA Kit, ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general inositol trisphosphate,Inositol trisphosphate
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Inositol trisphosphate, During the reaction, Inositol trisphosphate in the sample or standard
competes with a fixed amount of biotin-labeled Inositol trisphosphate for sites on a pre-coated Monoclonal antibody specific to Inositol
trisphosphate. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish
Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inositol trisphosphate in the samples is then determined
by comparing the O.D. of the samples to the standard curve

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