Canine Leukotriene C4, LTC4 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Leukotriene C4(LTC4);

Search name
Canine Leukotriene C4 ELISA Kit, Canine LTC4 ELISA Kit, Leukotriene C4 ELISA Kit, LTC4 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Leukotriene C4,LTC4 concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to LTC4, During the reaction, LTC4 in the sample or standard competes with a fixed amount of
biotin-labeled LTC4 for sites on a pre-coated Monoclonal antibody specific to LTC4. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTC4 in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

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