Canine PDE6B ELISA Kit
Product Name:Canine PDE6B ELISA Kit
Packing:96T
Catalog No.:ELI-45860d
Gene Name:Canine PDE6B
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine PDE6B
Alternative Name:PDE6B,PDE6B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine PDE6B ELISA Kit allows for the in vitro quantitative determination of Canine PDE6B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine PDE6B ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Canine PDE6B ELISA KIT,CSNB3 ELISA KIT,CSNBAD2 ELISA KIT,PDEB ELISA KIT,RP40 ELISA KIT,rd1 ELISA KIT, GMP-PDE beta phosphodiesterase 6B ELISA KIT, cGMP-specific rod beta rod cGMP-phosphodiesterase beta-subunit ELISA KIT,phosphodiesterase 6B ELISA KIT, Canine rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta ELISA KIT
Intended use
Canine PDE6B ELISA kit allows for the in vitro quantitative determination of Canine PDE6B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Canine PDE6B ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE6B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE6B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDE6B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Canine PDE6B ELISA kit Range 46.78-3000pg/ml
> PDE6B ELISA KITS
has been pre-coated with an Canine PDE6B antibody specific to Canine PDE6B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine PDE6B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine PDE6B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine PDE6B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!